hydrolysis-resistant polyacrylamide gels bio in chile ...
out on denaturing polyacrylamide gels (12% or 15% acrylamide, 0.75%bis-acrylamide, 7M urea; gel size: 40 x 30 x 0.04 cm in 50 mM Tris-borate pH 8.3, lmM EDTA at 1500-2000 V for approximately 2 and 5 hours for long and short runs, respectively.
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