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    2020-7-11 · Another low volume but important use of anionic and cationic polyacrylamides is gel electrophoresis for macromolecule separation. When an electic field is applied across a PAM gel, the (negatively) charged proteins or nucleic acids migrate across the gel away towards the positive electrode.

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    Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.Pierce 1.8K

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    Protein Electrophoresis Gels & Buffers. Polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE are common techniques used for protein separation. Protein gels can be hand-casted or purchased as pre-cast gels for convenience. The percentage of acrylamide in the gel affects resolution of protein bands, with higher percentages of acrylamide useful ...

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    Samples were resolved on polyacrylamide gel and then stained with Coomassie BB R-250 or transferred to a PVDF membrane for Western analysis. .. Anti-Hsp70 (Invitrogen, cat# PIPA534772) and anti-Gpd1 (Fisher, cat# PIPA572524) were used to probe the transferred proteins, and goat-anti-rabbit HRP (Fisher, cat# PI31460) was utilized as a secondary ...

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    2015-8-1 · A comprehensive review of polyacrylamide polymer gels for conformance control Baojun Bai 1, *, Jia Zhou 2 , Mingfei Yin 1 1. Missouri University of Science and Technology, Rolla 65409, USA; 2. BASF Corporation, Houston 77802, USA Abstract: Polymer gels have been designed and successfully applied to improve sweep efficiency and to reduce ...

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    Polyacrylamide gel system is widely been used in oilfield for water shutoff treatment. Gelation behavior such as proper gelation time provides a basic guideline on gel placement depth in reservoir layers. In this study, the gelation behavior of partially hydrolyzed polyacrylamide crosslinked with chromium(III) acetate (PHPA/Cr3+) reinforced with nano-silica was studied in term of gelation time ...

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    Protein polyacrylamide gel electrophoresis: (1)The basic principle of SDS denaturing in-continuous polyacrylamide gel electrophoresis is based on differences in the molecular weight of the protein, SDS is an anionic surfactant, capable of binding with the hydrophobic portion of the protein thus making the protein bring a large number of anions ...

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    Herein, a low cost and biocompatible fluorescent double network (DN) hydrogel based an anti-counterfeiting platform is demonstrated. The fluorescent hydrogels (agar/pAM–PCD DN gels) were composed of agar with a thermally reversible sol–gel transition property as the first physical network and polymerizable carbon dots (PCD) crosslinked ...

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    Polyacrylamide gel system is widely been used in oilfield for water shutoff treatment. Gelation behavior such as proper gelation time provides a basic guideline on gel placement depth in reservoir layers. In this study, the gelation behavior of partially hydrolyzed polyacrylamide crosslinked with chromium(III) acetate (PHPA/Cr3+) reinforced with nano-silica was studied in term of gelation time ...

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    Sensitive detection of proteins in polyacrylamide gel via isatoic anhydride derivatization: Introduction of a low-cost fluorescent prelabeling procedure. Asadollahi K(1), Rafiee S(1), Riazi G(2). Author information: (1)Neuro-organic Laboratory, Institute of Biochemistry and Biophysics (IBB), University of …

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    In order to construct the in vitro cell culture model occurring in biochemical and biophysical environment in vivo, and to establish quantitative detection and control of mechanical properties of substrate, a low modulus material polyacrylamide hydro-gel (PAHG) was synthesized and used as the substrate material for in vitro cell culture. Using a modified tensile device based on the principles ...

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    Cleaver Scientific’s multiSUB horizontal gel electrophoresis units have been designed by scientists with the laboratory environment in mind.Our multiSUB Horizontal Electrophoresis tanks provide an easy to use and flexible platform for all your horizontal electrophoresis requirements.With a wide range of tanks and tray sizes as well as many comb options, these systems can handle all manner of ...

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    Factory Supply Polyacrylamide. FOB Price:0.1 USD/Kilogram Min.Order:1 Kilogram Supply Ability:2000 Metric Ton/Year Appearance:solid or liquid ( refer to COA) Purity:99.90% Packaging:25kgs/fiber drum or 200kgs/drum Usage:upon client's application Brand:Amitychem Description:Amitychem Corporation manufacture and supply Polyacrylamide with with high quality, low price and bulk supply.

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    PAGE GelRed® Nucleic Acid Gel Stain. PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically formulated to stain DNA in polyacrylamide gels. PAGE GelRed® can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter. …

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    2011-6-17 · Gels provide a simple, low-cost way to separate nucleic acids based on size for quantification and purification. The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1).

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    Product Information. The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on ...

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    2019-12-19 · In PAGE Purification method, oligonucleotides are loaded on a polyacrylamide gel with the best appropriated percentage of acrylamide to purify the desired full-length oligonucleotide. The major product obtained is the slowest migrating band, which is excised out of the gel under low …

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    Blufloc Polyacrylamide (PAM) Polyelectrolyte is water soluble polymer with Anionic, Cationic and Nonionic types. The range of molecular weight is different, Anionic is from 5-22 million, Cationic is from 5-12 million and Nonionic is from 5-12 million, with a charge density ranging from 0-60%. Get Price.

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    2003-9-1 · The cost of using this system for genotyping is low. The gel ingredients cost approximately 2.60, and a gel can be used to obtain 100 data points without multiplexing. This brings the gel cost per data point to less than 0.03. The gel system itself is also inexpensive with a current price …

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    Polyacrylamide PAM, with the chemical formula of (C3H5NO)n and the CAS NO. of 9003-05-8, is a linear polymer. Polyacrylamide polymer has good thermal stability, can be dissolved in water in any proportion, and the aqueous solution is a uniform and transparent liquid. PAM is one of the most widely used water-soluble polymers.

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    ratory; however, the price of sequencing is expensive. In order to diagnose Gilbert's syndrome, it is important to establish a simple, effective and low cost method to detect mutations of the UGT1A1 gene. In this study, to the best of our knowledge, we applied the three-dimensional polyacrylamide gel-based

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