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    Gel Shift Assays–EMSA. The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One important technique for studying gene regulation and determining protein–DNA interactions is the electrophoretic mobility shift assay (EMSA).

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    Applications of Agarose Gel:-The main purpose of agarose gel is to isolate and analyze the DNA molecules which are cut by restriction enzymes. These cut pieces of DNA can be used for the cloning purpose to make various plasmids from one fragment. The function of gel electrophoresis is to isolate cut plasmids or vectors from the uncut vectors.

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    1987-5-15 · 4. DNA sieving in amylose-agarose gels. A three-part gel was prepared containing 0.7% agarose with differing concentrations of amylose. The gel was protected against leaching and the marker DNAs were applied as described in Fig. 3A. Data for agarose (•) [gel (a)], agarose plus 2% amylose (O) [gel (b)], and agarose plus 4% amylose (+) are provided.

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    2011-8-5 · or fluorescent molecules can disperse uniformly in the gel network of agarose (figures 1(b) and (c)). In preparing the thermally induced hydrogel, we incor-porated nonionic surfactants into the agarose network [21]. The principle of a thermally induced reversible hydrogel is straightforward. In the preparation of EPE hydrogel, hot

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  • A quick and effective in-house method of DNA

    2017-6-29 · Although our proposed change was simple (electrophoresis on 0.65% agarose gel and the use of 0.5× TAE instead of 1× TBE), the result was remarkable. The merit of this protocol is its simplicity, low cost, and suitability for multiple purposes; including, for the …

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    2018-4-20 · Figure 3: Sensitivity of the protocol as measured by 50-2000 bp DNA marker on a non-denaturing polyacrylamide gel. The first lane was loaded with 1µL of DNA marker with fragment sizes of 50, 100, 200, 300, 400, 500, 600, 700, 1000, 1500 and 2000 bp, each at 10 ng/µL. The remaining samples were loaded with a 1:2 serial dilution in preceding lanes.

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    2020-10-23 · The use of TAE buffer in a denaturing gradient gel electrophoresis method for broad-range mutation analysis has been described. TBE. TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp. TBE is used with both non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel.

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    2021-4-13 · Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surface. As hydrogels are highly biocompatible, in this study, we developed agarose and gelatin-based hydrogel films ...

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    2020-7-25 · Works pretty good with the green gel stain and 100 bp DNA ladder. Also good price. lowest cost good agarose Posted by Machiavelli on 23rd Oct 2018 I have run hundreds of gels with The ODIN branded agarose and it is satisfyingly low cost and pure. great price, great gels Posted by …

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  • UltraPure™ Low Melting Point Agarose

    UltraPure™ Low Melting Point Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Low Melting Point Agarose is ideal for resolving DNA and RNA fragments and for the recovery of nucleic acid fragments after electrophoresis, as it melts at 65.5°C (below the ...

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  • Gel Shift Assays (EMSA) | Thermo Fisher Scientific - US

    Gel Shift Assays–EMSA. The interaction of proteins with DNA is central to the control of many cellular processes including DNA replication, recombination and repair, transcription, and viral assembly. One important technique for studying gene regulation and determining protein–DNA interactions is the electrophoretic mobility shift assay (EMSA).

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  • Gel Imaging Systems | Life Science Research | Bio-Rad

    Learn about gel boxes, running buffers, agarose types, and other factors affecting resolution in DNA gel electrophoresis. 2-D Electrophoresis, Imaging, and Analysis. 2-DE is an essential step in protein characterization, purification and profiling, and posttranslational modification …

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  • A universal method for two-dimensional

    1991-8-1 · A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using ... Two-dimensional gel electrophoresis with immobilized pH gradients in the first dimension, initially applied for the separation of soluble and total cellular proteins, has been extended to the analysis of membrane proteins. ... The gel strips ...

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  • Slab-Gel Electrophoresis - an overview | ScienceDirect

    C.M. Roth, in Encyclopedia of Analytical Science (Second Edition), 2005 Materials. The two materials used most commonly for DNA slab gel electrophoresis are polyacrylamide and agarose. Polyacrylamide gels are formed with pore sizes that depend on the percentage of acrylamide used as well as the proportion of cross-linker, N,N′-methylene bisacrylamide.

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    The Emprove ® Program combines high-quality products, comprehensive documentation, and superior customer support to facilitate your efforts in qualification, risk assessment, and process optimization. It covers over 400 raw and starting materials, selected filter and single-use product families, chromatography resins, and cell culture media.

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  • Negative detection of biomolecules separated in ...

    2006-11-9 · Identical serial dilutions of total proteins from Escherichia coli (from 10 to 0.05 μl total protein extract) were separated in two 12.5% polyacrylamide-SDS minigels. (a) The gel was stained with ...

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    2021-6-16 · Our primary purpose is to give our shoppers a serious and responsible company relationship, giving personalized attention to all of them for Decolorant Polymer, Nonionic Polyacrylamide Pam, Anionic Polyacrylamide Flocculant, In case you pursuit the Hi-quality, Hi-stable, Aggressive price elements, corporation name is your greatest choice!Decolorant

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    2021-5-29 · electrophoresis—polyacrylamide and agarose. The support matrices act as porous media and behave like a molecular... Polyacrylamide has a smaller pore size and is ideal for separating most proteins and smaller nucleic acids. Polyacrylamide gel electrophoresis (PAGE) Polyacrylamide gels are generated by the polymerization of... For Free enquiry

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    2021-6-17 · The agarose method produced banding patterns and isoelectric points which corresponded to the polyacrylamide gel data for most samples. Differences were observed for HMS-1 and PSE-1 β-lactamases. The HMS-1 sample produced two highly resolvable enzyme bands in agarose gels rather than the single faint enzyme band observed on polyacrylamide gels.

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    This study have separated the fragments of Qinyou 33 hybrid and parent plants amplified by the three candidate primers SR85,SR332 and SR407 using agarose gel electrophoresis and polyacrylamide gel electrophoresis respectively.The comparative study showed that:(i) Using agarose gel electrophoresis,the bands of hybrid are complemented by the bands of parents amplified by the three candidate ...

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  • polyacrylamide and agarose gel electrophoresis

    2014-5-25 · Sodium dodecyl sufate polyacrylamide gel electrophoresis Special form of PAGE that employs a detergent to denature the protein. Proteins assume a rod like shape in the presence of SDS. The net result is that the proteins have similar shapes and charge-to-mass ratios and are therefore separated by gel filtration effects.

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  • Comparative biotolerance of polyacrylamide-agarose

    Comparative biotolerance of polyacrylamide-agarose gel, silicone rubber and microporous PTFE as soft tissue implants Author links open overlay panel David E. Taylor John E. Penhallow Show more

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    Smeared DNA bands in polyacrylamide gels, but not in agarose gel. Possible reasons? Tung T Le @Tung-Le-17. 20 November 2016 11 3K Report. Hi everyone, I ran some DNAs and saw that they looked normal on an agarose gel but very smeared on a polyarylamide gel. They smeared towards high molecualar weight band. I tried 0.5X or 0.25X TBE as running ...

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  • DIGITAL.CSIC: Comparison of SSR polymorphisms

    2020-5-20 · 7 pages, 1 figure, 2 tables. Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/18635

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  • NBS Biologicals LIFE SCIENCE Catalogue 2016

    RNA in agarose gel. This dye replaces Ethidium Bromide (toxic, potential ... • Can be used for Agarose and Polyacrylamide Gel Electrophoresis Ordering Information ... AB0015-25G Agarose, Low Melting 25g £81.00 OUR LOWEST COST AGAROSE FOR ROUTINE APPLICATIONS • Low EEO (0.09-0.13)

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  • A rapid and cost effective method in purifying small

    2011-6-3 · Purification of RNA fragments from a complex mixture is a very common technique, and requires consideration of the time, cost, purity and yield of the purified RNA fragments. This study describes the fastest method of purifying small RNA with the lowest cost possible, without compromizing the yield and purity. The technique describes the purification of small RNA from polyacrylamide gel ...

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  • Low-Cost Methods for DNA Extraction and

    2016-12-9 · In addition, modular 96-well rapid agarose gel electrophoresis such as the E-Gel 96 system (Thermo Fisher Scientific) can be loaded with multichannel pipettors and be used to electrophorese hundreds of samples in less than 10 min (see Fig. 14.3). Images from these gels are suitable for the quantification method described here.

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  • ACS Symposium Series (ACS Publications)

    Silver-Stain Detection of Proteins Separated by Polyacrylamide Gel Electrophoresis Carl R. Merril Chapter 5 , 74-90 DOI: 10.1021/bk-1987-0335.ch005 Publication Date (Print) : March 18, 1987

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  • Overview of Current Molecular Biology

    2021-2-5 · • Vertical native polyacrylamide gels • Gel filtration • Assembly of complexes • RNA is transcribed that contains 32 P. • Incubate under conditions that promote the formation of complexes • Complexes are run on an agarose gel. • Gels are visualized on a Phosphorimager. • Why study RNA-Protein complexes? • Protein misfolding

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    2018-4-3 · 44. Walczak R, Kubicki W, Dziuban J. Low cost fluorescence detection using a CCD array and image processing for on-chip gel electrophoresis. Sensor Actuat B-Chem. 2017;240:46-54 45. Danish A, Lee SY, Muller CE. Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis. Analyst. 2017;142:3648-55 46.

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  • An Optimization Study of Polyacrylamide

    2018-10-2 · The used polymer in this study is nonionic polyacrylamide (PAM) that was obtained from Sigma-Aldrich. It is in powder form with a pH of 6 and was used as provided. The molecular weight of PAM is with average 5,000,000–6,000,000 mol. wt. Polyethylenimine (PEI) was used as crosslinker provided by BASF with a pH of approximately 11.7.

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN

    2020-9-3 · PCR cycling used an initial Hot Start Taq activation at 95°C for 10 min followed by 35 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s with a final 10‐min extension at 72°C. PCR products for agarose gel detection (5 µl) were visualized with ethidium bromide on a 2% TAE agarose gel.

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    LE Agarose . Low Electroendosmosis (LE) Agarose is a high quality molecular biology grade Agarose suitable for analytical and preparative electrophoresis of nucleic acids. Nucleic acid separation with Agarose LE is between 0.2 - 15 kbp depending on the Agarose concentration.

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    2018-6-6 · (C) Resolution at 1 bp. Single-stranded DNA (ssDNA) oligomers were run on 3% agarose gels at 29 V/cm for 25 min. Each lane was loaded with a different size of oligomer mixed with a 35-oligomer. A TAE gel run under the same conditions melted (55°C after 20 min). A TBE gel was partially melted and showed fuzzy, unresolved bands (>50°C after 20 ...

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    Wuxi Fengmin Environmental Technology Development Co., Ltd., Experts in Manufacturing and Exporting Polyacrylamide, Super Absorbent Polymer and 1080 more Products. A Verified CN Gold Supplier on Alibaba.com.

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    Figure 3.5. Isoelectric focusing. A pH gradient is created in the gel (usually made of polyacrylamide, less frequently agarose). Upon electrophoresis, various proteins will accumulate in different narrow regions of the gel where the pH equals their individual pI value. If, in a given protein, the number of acidic residues (Asp, Glu) exceeds that of the basic ones (Arg, Lys, His), the protein ...

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    2021-3-12 · The study deals with the synthesis and characterization of the hydrophobically modified polyacrylamide (HMPA) copolymer and its functional property evaluation in mixed polymer-gemini surfactant systems for application in enhanced oil recovery (EOR). The copolymer was initially prepared in the laboratory using acrylamide and N-phenylacrylamide monomer units via an addition …

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    The use of cationic, anionic and nonionic polyacrylamide. Polyacrylamide (PAM) is a linear water soluble polymer, is a water-soluble polymer compounds used in wide varieties of one, PAM and its derivatives can be used as efficient flocculant, thickening agent, paper strengthening agent and liquid drag reduction agent, which is widely used in water treatment, paper, petroleum, coal, mining ...

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    2020-8-12 · analysis by 1% E-Gel agarose gel. The ChargeSwitch kit recovered DNA at higher yields and purity (A 260 /A 280), than the MagAttract and Agencourt AMPure kits. DNA purity as analyzed by agarose gel electrophoresis was high, and was comparable for all three kits. The ChargeSwitch kit provided the fastest protocol as well as the lowest price per ...

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    Agarose and polyacrylamide are polymers that meet these criteria. Agarose Gels o Agarose is a polysaccharide polymer extracted from seaweed comprised of agaropectin and agarose. Agarose is a linear polymer of agarobiose, which consists of 1,3-linked-β -d-galactopyranose and 1,4-linked 3, 6-anhydro- α -l-galactopyranose ( Fig. 4.2).

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    2018-10-2 · The used polymer in this study is nonionic polyacrylamide (PAM) that was obtained from Sigma-Aldrich. It is in powder form with a pH of 6 and was used as provided. The molecular weight of PAM is with average 5,000,000–6,000,000 mol. wt. Polyethylenimine (PEI) was used as crosslinker provided by BASF with a pH of approximately 11.7.

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    In addition, polyacrylamide gel can be divided into denaturing and non-denaturing polyacrylamide gels, and both can be used for the detection of SSR markers using the silver staining method. The effect and resolution of which do not significantly differ, but non-denaturing polyacrylamide gels are easier to process and take less time 16 .

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    Denaturing Polyacrylamide/Urea Gel Electrophoresis. and gel pieces. 10. Load the samples. 11. Run the gel at 6 V/cm till the lower dye front reaches the three thirds of the gel. 12. Soak the gel for about 15 min in 1X TBE to remove the urea prior to staining. 13. Stain the gel in a 0.5 碌g/ml ethidium bromide aqueous solution for about 30 min. 14.

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    The use of cationic, anionic and nonionic polyacrylamide. The use of cationic, anionic and nonionic polyacrylamide. 2016-01-16 08:46:38. Polyacrylamide (PAM) is a linear water soluble polymer, is a water-soluble polymer compounds used in wide varieties of one, PAM and its derivatives can be used as efficient flocculant, thickening agent, paper strengthening agent and liquid drag reduction ...

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    2018-10-2 · The used polymer in this study is nonionic polyacrylamide (PAM) that was obtained from Sigma-Aldrich. It is in powder form with a pH of 6 and was used as provided. The molecular weight of PAM is with average 5,000,000–6,000,000 mol. wt. Polyethylenimine (PEI) was used as crosslinker provided by BASF with a pH of approximately 11.7.

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  • ACS Symposium Series (ACS Publications)

    Silver-Stain Detection of Proteins Separated by Polyacrylamide Gel Electrophoresis Carl R. Merril Chapter 5 , 74-90 DOI: 10.1021/bk-1987-0335.ch005 Publication Date (Print) : March 18, 1987

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    2017-1-1 · geometric information about a particular gel-solute system. Comparisons with data taken in agarose gels, provided by Clague and Phillips (1996), Johnson et al. (1996), and Pluen et al. (1999), combined with the comparisons with poly-acrylamide, alginate, and carrageenan gels given here, dem-onstrate that Eq. 8 captures much of the basic physics ...

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    CHAPS (formal name: 3-cholamidopropyl dimethylammonio 1-propanesulfonate) is a non-denaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. This detergent combines the useful properties of both sulfobetaine-type and the bile salt detergents. CHAPS is commonly used for protein solubilization ...

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    3.2 Polyacrylamide Gel Electrophoresis. Polyacrylamide gels are formed by copolymerization of acrylamide monomer, CH 2 = CH-CONH 2, and a cross-linking comonomer, N,N′-methylenebi-sacrylamide, CH 2 = CH-CO-NH-CH 2-NH-CO-CH = CH 2 (bisacrylamide).Mechanism of Gel Formation. The mechanism of gel formation is vinyl addition polymerization and is catalyzed by a free radical …

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    2014-1-30 · SDS polyacrylamide gel electrophoresis (SDS PAGE), perhaps the most ubiquitous benchtop fractionation technique in use today, separates and orders proteins according to their apparent molecular weight.Yet in complex mixtures like cell lysates, many different proteins may have the same electrophoretic mobility and so a second, orthogonal technique often is required.

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    2001-5-15 · In non-denaturing electrophoresis, a buffered solution of native proteins is poured onto a porous gel (usually polyacrylamide, starch or agarose) and a voltage is applied across the gel. The proteins move through the gel in a direction that depends on the sign of their charge, and at a rate that depends on the magnitude of the charge, and the ...

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    2021-7-6 · Calculate the volume of gel-ready sample to load in each well. Now that you know the concentration of the sample you'll be loading on the gel, you can calculate the volume to pipet into the well. The recommended mass of protein may vary for different experiments, but for your first gel with fish muscle proteins, try to get 4 μg protein in each ...

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  • Protein Gel Staining Methods | Thermo Fisher

    Example gel stained with a zinc stain. A 2-fold dilution series of a protein mixture was separated by protein gel electrophoresis using a 15-well mini gel. Subsequently the gel was stained using the Thermo Scientific Pierce Zinc Reversible Stain Kit, and then photographed with the gel placed over a dark blue background. The sensitivity on this ...

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  • Pippin HT - Labtech

    More Details. Building on the success of the Pippin Prep and Blue Pippin size selection technologies, the PippinHT provides the user with the ability to size select up to 24 samples. Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read (including ...

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  • These 2D Gel Tools Will Have You Seeing (Well

    2014-1-30 · SDS polyacrylamide gel electrophoresis (SDS PAGE), perhaps the most ubiquitous benchtop fractionation technique in use today, separates and orders proteins according to their apparent molecular weight.Yet in complex mixtures like cell lysates, many different proteins may have the same electrophoretic mobility and so a second, orthogonal technique often is required.

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  • Low Cost Nylon Membrane Filters - Samples Available ...

    Nylon membrane filters are flexible, durable and tear resistant, and can be autoclaved at 135°C. If you are using nylon 66 membrane filters then give us a call so we can ship samples to compare with our Nylon Membrane. Tisch Scientific offers an extensive selection of proven, yet affordable products that are in stock and ready to ship today.

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  • Western Blot - StatPearls - NCBI Bookshelf

    2021-4-28 · The name, ‘western’ blot, was first coined by Dr. Burnette in 1981 after the eponymous southern blot for DNA and consequent coinage of the northern blot in 1977 for RNA.[1][2] The western blot (WB) is an effective and widely utilized immunoassay that confers selective protein expression analysis. WB selects for an individual protein amongst a potentially significant milieu via leveraging ...

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  • Calcium sensitivity of the contractile system and ...

    The proteins of the tissue were then isolated by SDS-polyacrylamide gel electrophoresis, and the identity of 32P-labeled proteins was determined. The Ca sensitivity is inversely related to the relative amount of 32P incorporated into the inhibitory subunit of troponin (TNI).

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  • Optimization and Efficient Purification in Production

    2013-9-1 · The development of an effective subunit vaccine against brucellosis is a research area of intense. But optimization of recombinant proteins production in Escherichia coli and content of endotoxins associated with final recombinant proteins are very important.In the present study, expression and purification of Brucella melitensis rHSP and rTF were optimized to reduce endotoxin contaminants ...

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  • Taq DNA Polymerase - QIAGEN

    Taq DNA Polymerase is a high-quality recombinant enzyme that is suitable for general and specialized PCR applications (see figures ' Tolerance of different primer T m Values' and ' Specific amplification of long PCR products ').. QIAGEN PCR Buffer . Innovative QIAGEN PCR Buffer has been developed to save time and effort by reducing the need for PCR optimization.

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  • Electrophoresis & Immunoblotting Flashcards | Quizlet

    Run agarose gel electrophoresis to separate the fragments based on size and transfer the results onto a polymer sheet 3. A specific restriction fragment of interest can be detected by creating and adding a ___________ to the sheet.

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  • Proteins, Electrophoresis, and Lipids Flashcards | Quizlet

    Sodium dodecyl sulfate (SDS) is a nonionic detergent that binds to proteins, neutralizing their charge. Polyacrylamide gel electrophoresis (PAGE) after treating with SDS separates proteins on the basis of molecular size. The smaller proteins become trapped in the pores of the gel and migrate more slowly.

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