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starch-g-polyacrylamide in france

  • hydrolyzed polyacrylamide grafted maize starch

    Cassava starch-g-polyacrylamide (PAM) was successfully prepared by a reactive batch processing using a specially designed batch reactor resulting in a superabsorbent polymer (SAP) with water absorption of 605 g/g being obtained under the optimized reaction conditions.The occurrence of a graft copolymer was confirmed by FT-IR spectra, where it was found to exhibit all characteristic bands of

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  • Dual starch–polyacrylamide polymer system for

    2017-11-1 · A starch–PAM system reduces PAM usage for conventional and ballasted flocculation. • With 0.4 mg starch/L, PAM is reduced by 69% (surface water) and 45% (wastewater). • With the dual system (G: 165-200 s −1), floc resistance is not impacted. • Coaddition of a starch-PAM blend solution could be used to reduce the use of PAM.

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  • Controlled release of phosphorous fertilizer bound to ...

    2018-9-1 · A controlled release fertilizer system based on phosphate bound-carboxymethyl starch-graft-polyacrylamide (P-CMS-g-PAM) has been prepared in order to deliver the phosphate fertilizer to the plant at a constant rate thereby. This system aims to increase fertilizer phosphorus use efficiency (FPUE) and maintain a hydration level for the plant at the same time.

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  • Functions of Heteromeric and Homomeric Isoamylase

    d'Ascq cedex 59655, France (S.G.B.) Functions of isoamylase-type starch-debranching enzyme (ISA) proteins and complexes in maize (Zea mays) endosperm were characterized. Wild-type endosperm contained three high molecular mass ISA complexes resolved by gel permeation chromatography and native-polyacrylamide gel electrophoresis.

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  • Acrylamide copolymers: A review of methods for the ...

    1994-12-1 · The starch-iodide method, as modified for flow injec- tion analysis, is useful for the determination of acrylam- ide copolymers in process streams, surface waters and oilfield brines. Limitations of the method include sen- sitivity to polymer degree of hydrolysis, and interfer- ences from iodide and amines.

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  • Starch makes a comeback - 2021 - Wiley Analytical

    2021-3-15 · Scientists from Ghana and the US are exploring the potential of replacing expensive agarose and polyacrylamide electrophoresis gels with cheaper gels made from corn starch. The largest repository of validated, free and subject-focused e-publications and online seminars in analytical science covering latest techniques, equipment, original research, editorials, and industry news and trends.

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  • IJMS | Free Full-Text | Synthesis and Characterization ...

    Preparation of Soluble Starch-G-Poly (Acrylic Acid-Co-2-Hydroxyethylmethacrylate) We mixed 2.0149 g of soluble starch and 35 mL of deionized water in a 250 mL round bottomed flask. The system was heated to 80 °C by the heating mantle and stirred with a mechanical stirrer at a …

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  • Rheological characterization and performance of ...

    2021-1-1 · Starch and Polyacrylamide: IOTs 53% Fe 2 O 3, 21% kaolinite, 15% goethite: Polyacrylamide is poorly selective. Wheat starch best recoveries (80%) at pH between 9 and 11: Potato and Corn Starch: IOTs 60% Fe 2 O 3, 20% SiO 2, 5% Al 2 O 3, 8% CaO: The presences of …

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  • Alpha-1-Antitrypsin phenotypes in Lyon, France |

    The agreement of the results obtained by our technique and by acid-starch gel electrophoresis confirms the validity of Pi determination by thin-layer isoelectric focusing. The variants were conf Alpha-1-Antitrypsin phenotypes in Lyon, France | SpringerLink

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  • Soluble starch synthase I: a major determinant for the ...

    2005-7-5 · The homogenate was filtered through two layers of Miracloth and centrifuged for 15 min at 4°C and 4000 g. The pellet was resuspended in 30 ml Percoll 90% (v/v) and centrifuged for 40 min at 4°C and 10 000 g. The starch pellet was washed six times with distilled sterile water (10 min at 4°C and 10 000 g between each wash). Starch was finally stored at 4°C in 20% ethanol.

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  • hydrolyzed polyacrylamide grafted maize starch

    Hydrolyzed polyacrylamide grafted maize starch based FTIR spectra of maize starch (MS), acrylamide grafted maize starch (Pam-g-MS), and hydrolyzed polyacrylamide grafted maize starch (HPam-g-MS). In the spectrum of Pam-g-MS the peaks at 3634.01 cm −1 , 3399.45 cm −1 and 3206.08 cm −1 are observed, which can be assigned to the overlap of N ...

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  • Synthesis and properties of

    2000-10-19 · A novel starch‐graft‐polyacrylamide/clay superabsorbent composite is synthesized by graft copolymerization reaction of acrylamide, potato starch and clay mineral micropowder, followed by hydrolysis with sodium hydroxide.The water absorbency of the composite reaches 4 000 g H 2 O/g. The influence of kind and amounts of clay and of the crosslinker in the composite on the absorbent …

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  • Synthesis and Properties of Polyacrylamide‐Starch

    Hossein Mahdavi, Ozra Rahmani, Polyacrylamide-g-Reduced Graphene Oxide Supported Pd Nanoparticles as a Highly Efficient Catalyst for Suzuki–Miyaura Reactions in Water, Catalysis Letters, 10.1007/s10562-016-1851-1, 146, 11, (2292-2305), (2016).

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  • Reactive Extrusion of Starch–Polyacrylamide Graft ...

    2006-3-18 · Graft copolymers of polyacrylamide and various substrates were prepared by reactive extrusion in a twin screw extruder using ammonium persulfate as initiator. Substrates included unmodified starches (corn, waxy maize, wheat, and potato), cationic starches, dextrin, dextran, and polyvinyl alcohol (PVOH). The feed ratio of substrate to monomer was 2:1. Average conversion of monomer to polymer ...

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  • Inverse Emulsion of Starch‐graft‐Polyacrylamide - Lu ...

    2003-5-6 · An inverse emulsion of cationic starch‐graft‐polyacrylamide was prepared by inverse emulsion polymerization and a subsequent Mannich reaction. The copolymerization was carried out using potassium persulfate as initiator. The reaction conditions and factors affecting emulsion stability were studied. Experiments showed that a high solids ...

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  • Synthesis, characterization and evaluation of ...

    2017-11-16 · remove any impurities and dried overnight at 60 C. Starch (St) graft polyacrylamide (PAAm-g-St) was also synthe-sized by the same procedure as above in the absence of nanoclay. The high amount of MMT ([2000 ppm) hin-dered the polymerization process, and the high amount of starch ([2.0 g) resulted in a low product yield. Finally,

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  • Controlled release of phosphorous fertilizer bound to ...

    2018-9-1 · A controlled release fertilizer system based on phosphate bound-carboxymethyl starch-graft-polyacrylamide (P-CMS-g-PAM) has been prepared in order to deliver the phosphate fertilizer to the plant at a constant rate thereby.This system aims to increase fertilizer phosphorus use efficiency (FPUE) and maintain a hydration level for the plant at the same time.

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  • Acrylamide copolymers: A review of methods for the ...

    1994-12-1 · Meadows et al. ( 1988, 1989) spin-labelled partially hydrolyzed polyacrylamide and measured its adsorp- tion onto cationic polystyrene latex. The use of ESR also allowed them to assess the configuration of adsorbed polymer molecules. Meadows et al. used a 90% hydrolyzed polyacrylamide, and substituted one spin label for every 64 carboxylate groups.

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  • Purification and characterization of an extracellular α ...

    2000-9-1 · MRS-medium (with glucose or soluble starch as substrates at 15 g/l) was routinely used for cultivation of L. manihotivorans. For amylase production, cells were grown with soluble starch (Prolabo, Paris, France) as substrate in 2-1 bioreactors (Biolafitte, Poissy, France) with pH controlled at 6 …

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  • The Pi System: Its Study by means of Thin-Layer-Gel ...

    1975-1-1 · An analytical technique, using thin-layer-gel electrofocusing in polyacrylamide gel, has been applied to the study of plasma α 1-AT polymorphism (Pi system).. With pH ranging from 4.5 to 5.5, we obtained at least eight bands which belong to the α 1-AT system, as confirmed by crossed immunoelectrophoresis of these bands with a specific antiserum.. As with classical acid starch-gel ...

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  • starch-grafted-polyacrylamide base

    Preparation of cassava starch-graft-polyacrylamide. Cassava starch-g-polyacrylamide (PAM) was successfully prepared by a reactive batch processing using a specially designed batch reactor resulting in a superabsorbent polymer (SAP) with water absorption of 605 g/g being obtained under the optimized reaction conditions.The occurrence of a graft copolymer was confirmed by FT-IR spectra, where it ...

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  • Synthesis and properties of

    2000-10-19 · A novel starch‐graft‐polyacrylamide/clay superabsorbent composite is synthesized by graft copolymerization reaction of acrylamide, potato starch and clay mineral micropowder, followed by hydrolysis with sodium hydroxide.The water absorbency of the composite reaches 4 000 g H 2 O/g. The influence of kind and amounts of clay and of the crosslinker in the composite on the absorbent …

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  • Performance Test of Starch-g-Polyacrylamide

    Performance Test of Starch-g-Polyacrylamide Synthesized through Grafting as a Flocculant in Artificial Wastewater Treatment Flocculation is one of the steps to get clean water. Grafted flocculant was successfully synthesized by combining the benefits of synthetic polymers (polyacrylamide) and natural polymers (cassava starch).

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  • activated starch as an alternative to polyacrylamide

    (PDF) Activated starch as an alternative to polyacrylamide-based polymers for in-line filtration of low turbidity source water. Since polymer cost has been reported to be a minimal proportion of the total chemical cost at a water treatment plant, activated starch may be a promising alternative to polyacrylamide.

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  • Microwave assisted synthesis of polyacrylamide

    2011-1-1 · Polyacrylamide grafted starch (St-g-PAM) was made by a novel method of synthesis, involving combination of microwave radiation and a chemical free radical initiator (ceric ammonium nitrate) to initiate grafting reaction. This method (microwave assisted synthesis) is quick, highly reliable, reproducible and yields high quality product as ...

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  • Preparation and Adsorption Properties of Starch-g ...

    By means of solution polymerization, a series of cross-linked starch-g-polyacrylamide montmorillonite (St-g-PAM/MMT) nanocomposites were prepared and used to adsorb methylene blue (MB). The effects of different preparation conditions on the adsorption capacity of the nanocomposites were investigated. The experimental results demonstrated that the prepared St-g-PAM/MMT nanocomposites were ...

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  • Functions of Heteromeric and Homomeric Isoamylase

    d'Ascq cedex 59655, France (S.G.B.) Functions of isoamylase-type starch-debranching enzyme (ISA) proteins and complexes in maize (Zea mays) endosperm were characterized. Wild-type endosperm contained three high molecular mass ISA complexes resolved by gel permeation chromatography and native-polyacrylamide gel electrophoresis.

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  • Thermo-Responsive Starch-g-(PAM-co-PNIPAM):

    Besides this, an interesting significant thermo-thickening behavior was also observed on highly branched starch-g-polyacrylamide at high temperatures (>80 °C), which has not been previously reported. Rheology tests also revealed a good salt-resistant property in copolymers with low NIPAM content (e.g…

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  • Assessment of corn starch as substitute for agarose in

    2018-10-1 · Application of starch grafted acrylamide in paper-making wastewater treatment was studied. 研究了淀粉接枝聚丙烯酰胺对造纸废水的处理,探讨了絮凝剂的投入量、废水的PH值、絮凝温度和絮凝时间对絮凝效果的影响,并得出了淀粉接枝聚丙烯酰胺作为絮凝剂处理脱墨废水时的最佳絮凝条件:絮凝剂用量为10mL,絮凝温度为40 ...

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  • EP0147380A2 - Functionalized polyacrylamide grafted

    @ A functionalised polyacrylamide grafted starch polymer useful as a paper wet strength additive wherein at least 5 weight percent of the corresponding polyacrylamide grafted starch polymer is polyacrylamide and comprising substituents derived from one or more materials containing two or more aldehyde units, e.g…

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  • Full-scale performance of selected starch-based ...

    2017-8-29 · The general feasibility of applying starch-based ‘green’ polymers in full-scale centrifugation was demonstrated. Depending on the sludge type and the process used, the substitution potential was up to 70%. Substitution of 20–30% of the polyacrylamide (PAM)-based polymer was shown to achieve similar total solids (TS) of the dewatered sludge.

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  • Maize Starch-g-poly(n-vinylimidazole) Synthesis and its ...

    2019-7-12 · Polyacrylamide grafted starch (St-g-PAM) has been synthesized by microwave assisted method involving a synergism of microwave radiation and ceric ammonium nitrate (CAN) to initiate the grafting reaction. The higher the Maize Starch-g-poly(n-vinylimidazole) Synthesis and its Application in

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  • DIREcT Gc DETERMINATION Of AcRyLAMIDE IN wATER

    2019-1-26 · 2 Acrylamide (C3H5NO) is a polar, low MW molecule and its analysis by direct GC/MS is not an easy task, mainly because of low peak efficiency in chromatographic separation and no-significant low mass fragmentation in MS detection [3].

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  • Preparation and Adsorption Properties of Starch-g ...

    By means of solution polymerization, a series of cross-linked starch-g-polyacrylamide montmorillonite (St-g-PAM/MMT) nanocomposites were prepared and used to adsorb methylene blue (MB). The effects of different preparation conditions on the adsorption capacity of the nanocomposites were investigated. The experimental results demonstrated that the prepared St-g-PAM/MMT nanocomposites were ...

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  • Hyper-accumulation of starch and oil in a

    2016-3-8 · Starch and oil hyper-accumulate in std1 mutant following N deprivation.a Starch content in cells grown photoautotrophically under low light (LL, 35 µmol photons m −2 s −1) supplemented with 2 % CO 2. b Starch content in cells grown photoautotrophically under medium light (100 µmol photons m −2 s −1) supplemented with 2 % CO 2. c Starch content in cells grown mixotrophically (i.e ...

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  • Starch Granule Biosynthesis in Arabidopsis Is

    Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules are still synthesized, albeit with altered amylopectin structure.

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  • Microwave assisted synthesis of polyacrylamide

    2011-1-1 · Polyacrylamide grafted starch (St-g-PAM) was made by a novel method of synthesis, involving combination of microwave radiation and a chemical free radical initiator (ceric ammonium nitrate) to initiate grafting reaction. This method (microwave assisted synthesis) is quick, highly reliable, reproducible and yields high quality product as ...

    Get Price
  • Granule-bound starch synthase (GBSS) diversity of

    Granule-bound starch synthase of ancient wheat and related species was examined by sodium dodecyl sulphate polyacrylamide gel. A total of 13 different alleles were revealed in a collection of three accessions of diploid wheat, six accessions of tetraploid wheat, 49 accessions of spelt wheat, nine accessions of Sitopsis and two accessions of Aegilops tauschii.

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  • Foodchem Group Ltd, Shanghai Honghao

    Constantly strive towards: High quality products Professional support Total solution for food Reasonable price Credible friendly cooperation

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  • Congruence between starch gel and polyacrylamide

    2003-2-1 · Taxonomic and population genetic re-interpretation of two color morphs of the decollate snail, Rumina decollata (Mollusca, Pulmonata) in southern France. Prévot V, Jordaens K, Van Houtte N, Sonet G, Janssens K, Castilho R, Backeljau T. Genetica, 141(7-9):281-292, 26 Jul 2013

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  • STARCH/POLYACRYLAMIDE GRAFT COPOLYMERS – THE

    To receive starch graft copolymers, acrylamide (AAm) ( 99%, Fluka) and [2-(acryloyloxy)ethyl]trimethylammonium chloride (AETAC) (water solution, Ciba Specialty Chemicals Ld.) monomers were used. Potato starch (superstand-ard quality) was obtained from “Nowamyl” S.A., Poland and corn starch from In-dustrial Enterprise “Roquette”, France.

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  • activated starch as an alternative to polyacrylamide

    (PDF) Activated starch as an alternative to polyacrylamide-based polymers for in-line filtration of low turbidity source water. Since polymer cost has been reported to be a minimal proportion of the total chemical cost at a water treatment plant, activated starch may be a promising alternative to polyacrylamide.

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  • PEMBUATAN FLOKULAN STARCH-g-POLYACRYLAMIDE ...

    2017-11-28 · TUGAS AKHIR TK141581 PEMBUATAN FLOKULAN STARCH-g-POLYACRYLAMIDE DENGAN GRAFTING TO Akhmat Fauzi NRP 23121 100 152 Dosen Pembimbing Dr. Ir. Sumarno, M.Eng NIP. 1964 06 08 1991 02 1001 Prida Novarita T., ST., MT

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  • Garuda - Garba Rujukan Digital

    garuda garba rujukan digital. Performance Test of Starch-g-Polyacrylamide Synthesized through Grafting as a Flocculant in Artificial Wastewater Treatment Kaavessina, Mujtahid; Fatimah, Ina; Soraya, Suci Equilibrium Journal of Chemical Engineering Vol 2, No 1 (2018): Volume 2 No 1 January 2018

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  • Purification and physicochemical properties of starch ...

    2019-3-17 · Starch phosphorylase from young banana leaves has been purified to homogeneity, as tested by disc polyacrylamide gel electrophoresis at various pHs and gel concentrations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunodiffusion, and immunoelectrophoresis, using the techniques of ammonium sulfate fractionation, DEAE-cellulose ...

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  • EnzymicMechanism of Starch Breakdown in

    ® soluble starch (i) P-limit dextrin FIG. 1. Amylase zymogram ofwhole rice seed extracts (4 d) separated by polyacrylamide gel isoelectric focusing. a and c, Isoelectric focusing combinedwith immunoabsorption using nonimmunecontrol rabbit serum. bandd, Isoelectric focusingcombinedwith immunoabsorption using …

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  • Genetic dissection of floridean starch synthesis in the ...

    2021-4-15 · 1 Biological sciences ‐ Biochemistry Genetic dissection of floridean starch synthesis in the cytosol of the model dinoflagellate Crypthecodinium cohnii David Dauvillée†, Philippe Deschamps†, Jean‐Philippe Ral*, Charlotte Plancke†, Jean‐Luc Putaux§, Jimi Devassine†, Amandine Durand‐Terrasson§, Aline Devin† and Steven G. Ball†,∏

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  • Genetic dissection of floridean starch synthesis in the ...

    2009-12-15 · Starch defines an insoluble semicrystalline form of storage polysaccharides restricted to Archaeplastida (red and green algae, land plants, and glaucophytes) and some secondary endosymbiosis derivatives of the latter. While green algae and land-plants store starch in plastids by using an ADP-glucose-based pathway related to that of cyanobacteria, red algae, glaucophytes, cryptophytes ...

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  • Reactive Extrusion of Starch–Polyacrylamide Graft ...

    for the starch substrates were approximately 25% with grafting efficiencies of about 70%. Polyacrylamide graft molecular weights ranged from 317,000 to 769,000. Absorbencies at pH 7 for saponified graft copolymers prepared with unmodified starches were approximately 200 g/g and approximately 150 g/g for the cationic star-ches, dextran, and PVOH.

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  • Study on Adsorption of Methylene Blue by Sarch-g ...

    By means of grafting polymerization, cross-linked starch-g-polyacrylamide (starch-g-PAM) was prepared and used to adsorb methylene blue (MB). Adsorption isotherm and kinetic of the sorption process were studied. The experimental results demonstrated that the prepared starch-g-PAM was an effective adsorbent for removal of MB from aqueous solution.

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  • US4105824A - Starch-dextrine-polyacrylamide

    Adhesive composition comprising starch, dextrine and a polyacrylamide. The composition is carried by water; but, when the composition is applied to a substrate, the water is evaporated to produce a water activatable adhesive. Thus, the composition is suitable for application to a paper backing to form an adhesive tape. Method of preparing the adhesive from a dry polyacrylamide starting ...

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  • Industrial Markets For Starch - Bangor University

    2006-11-26 · 8. Use of potato starch as a water co-flocculant is being promoted in some countries of continental Europe, on the basis that polyacrylamide, the most cost-effective co-flocculant, may not be safe. As this cannot presently be proved, polyacrylamide restriction policy is cautionary. Therefore, an environmental/health study of the use of

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  • Screening for natural inhibitors of penicillinase by ...

    2020-9-3 · The 0.08% hydrolyzed starch or glycogen were copolymerized in 7.5% or 10% sodium dodecylsulfate polyacrylamide gels. After electrophoresis and SDS removal, the commercial penicillinase in gels was reacted with penicillin G (100 mg in 50 mL, 0.1 M phosphate buffer, pH 7.0) for 30 min and then stained with 0.6% I 2 in 6% KI solutions.

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  • Biodegradation of low molecular weight

    2020-1-15 · Polyacrylamide (PAM), particularly high molecular PAM (>10 7 Dal), with a viscosity of over 58 centipoise (cp), has been increasingly used for enhanced oil recovery in oilfields ().Produced water generated during enhanced oil recovery contains high concentration of PAM, which should be removed before discharge (Yan et al. 2016).There have been a lot of studies focusing on the biodegradability ...

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  • Nature of the Periplastidial Pathway of Starch Synthesis ...

    tracted by denaturing 50 &g of starch granules in 80 &l of denaturating solution (2% sodium dodecyl sulfate [SDS] and 5% '-mercaptoethanol) for 10 min. The samples were centrifuged for 20 min at 10,000 g, and then supernatants were loaded onto a 7.5% SDS-polyacrylamide gel. …

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  • Screening for natural inhibitors of penicillinase by ...

    2020-9-3 · The 0.08% hydrolyzed starch or glycogen were copolymerized in 7.5% or 10% sodium dodecylsulfate polyacrylamide gels. After electrophoresis and SDS removal, the commercial penicillinase in gels was reacted with penicillin G (100 mg in 50 mL, 0.1 M phosphate buffer, pH 7.0) for 30 min and then stained with 0.6% I 2 in 6% KI solutions. The clear ...

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  • PERSONAL CARE - SNF

    2018-7-30 · SNF, headquartered in Andrézieux, France, is a leading global producer of water-soluble polymers with manufacturing sites in Andrézieux, France; Savannah, USA; and Taixing, China. For 2017, SNF had annual revenues of approximately 2.5G€ (2.71B). Over the last 40 years, SNF has developed expertise in polymer chemistries

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  • [Starch gel electrophoresis] - Abstract - Europe PMC

    [Electrophoresis with specific adsorption: amylase-starch interactions in mixed polyacrylamide-starch gel] Szylit M. Ann Biol Clin (Paris), 29(3):215-227, 01 Jan 1971 Cited by: 2 articles | PMID: 5564168

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  • Full-scale performance of selected starch-based ...

    2017-8-29 · The general feasibility of applying starch-based ‘green’ polymers in full-scale centrifugation was demonstrated. Depending on the sludge type and the process used, the substitution potential was up to 70%. Substitution of 20–30% of the polyacrylamide (PAM)-based polymer was shown to achieve similar total solids (TS) of the dewatered sludge.

    Get Price
  • Starch Granule Biosynthesis in Arabidopsis Is

    Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules are still synthesized, albeit with altered amylopectin structure.

    Get Price
  • (PDF) The use of isoelectric focusing on

    TKANSACTIONS OF THE ROYAL Socmu OF TROPICAL MEDICINE AND HYGIENE (1994) 88, 475-478 475 The use of isoelectric focusing on polyacrylamide gel for the enzymatic analysis of ‘Old World’ Leishmania species R. Piarroux, V. Trouv6, F. Pratlong, A. Martini, M. Lambert and J. A. Rioux Laboratoire d’Ecologie Medicale et Pathologic Parasitaire, Faculte de M&de&e, Universite de …

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  • The use of isoelectric focusing on polyacrylamide gel

    Using isoelectric focusing (IEF), a high resolution electrophoresis technique, we analysed 6 enzymes of 24 cloned strains representing all major taxa of 'Old World' Leishmania. The comparison of enzymatic patterns obtained with IEF and starch gel electrophoresis showed that IEF is a more discriminatory and more informative technique for the enzymatic analysis of Leishmania strains; it can ...

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  • Microscopic Study of Starch Gelatinization under High ...

    2021-3-12 · Starch gelatinization corresponds to a melting phase transition in aqueous medium. Such a transition involves important mass transfer of water. Using a high-pressure bomb including optical ports, the volume variation of starch granules in suspension was related to gelatinization during a high-pressure treatment up to 420 MPa. Microscopic observations of wheat and potato starch granules were ...

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  • Starch-binding domain affects catalysis in two ...

    2005-1-1 · A new starch-binding domain (SBD) was recently described in α-amylases from three lactobacilli (Lactobacillus amylovorus, Lactobacillus plantarum, and Lactobacillus manihotivorans).Usually, the SBD is formed by 100 amino acids, but the SBD sequences of the mentioned lactobacillus α-amylases consist of almost 500 amino acids that are organized in tandem …

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  • The influence of alterations in ADP-glucose ...

    Extraction of starch-granule-bound proteins and separation by SDS PAGE. Purified starch granules (20 mg) were heated at 95 °C for 5 min in sample buffer (Laemmli 1970) before being centrifuged at 10000 g for 5 min. Supernatant (50 |il) from each sample was loaded onto a 10% Polyacrylamide gel containing SDS and run at a constant 100 V for 2 h.

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  • Alpha-Amylase Starch Binding Domains: Cooperative

    2007). Starch binding domains (SBDs) belong to families CBM20, CBM21, CBM25, CBM26, CBM34, CBM41, and CBM45. Approximately 10% of all amylolytic enzymes possess a dis-tinct domain that facilitates binding to raw starch (13). This independent domain has been identified in such microorgan-isms as filamentous fungi, gram-positive bacteria ...

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  • o l o g y : OpenAc h e ces R Rheology: Open Access

    2017-12-29 · Starch-polyacrylamide graft copolymer and polyacrylamide production Starch was gelatinized in respective solvents by heating a starch suspension (6.5% solids) to 90°C and maintaining for 30 min. After gelatinization, the gelatinized starch solution was purged overnight with argon. Acrylamide (1.3 g) was added to the gelatinized starch solution,

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  • Dextran - FTIR - Spectrum - SpectraBase

    STARCH-G-POLYACRYLAMIDE: Source of Sample: I. K. Varma, Indian Institute of Technology, Centre for Material Science and Technology, New Delhi, India: Classification: MISCELLANEOUS CARBOHYDRATE DERIVATIVES: Comments

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  • Characterization of a Granule-Bound Starch Synthase ...

    1998-10-1 · Starch granule-bound proteins were separated by SDS-PAGE using 10% polyacrylamide gels (Laemmli, 1970). To extract starch granule-bound proteins the purified starch was suspended in SDS buffer and heated in boiling water. The starch solution was cooled on ice and centrifuged at 15,000g for 10 min, and the supernatant was subjected to SDS-PAGE ...

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  • Frontiers | Proteome Analysis of Potato Starch Reveals

    2017-8-29 · The general feasibility of applying starch-based ‘green’ polymers in full-scale centrifugation was demonstrated. Depending on the sludge type and the process used, the substitution potential was up to 70%. Substitution of 20–30% of the polyacrylamide (PAM)-based polymer was shown to achieve similar total solids (TS) of the dewatered sludge.

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  • Full-scale performance of selected starch-based ...

    Extraction of granule-bound starch proteins, SDS-PAGE, and Western blotting. g, and then supernatants were loaded onto a 7.5% SDS-polyacrylamide gel. For ADP-glucose pyrophosphorylase, the equivalent of 20 μg of total protein was separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were separated by SDS-PAGE according ...

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  • Nature of the Periplastidial Pathway of Starch

    2020-1-15 · Polyacrylamide (PAM), particularly high molecular PAM (>10 7 Dal), with a viscosity of over 58 centipoise (cp), has been increasingly used for enhanced oil recovery in oilfields ().Produced water generated during enhanced oil recovery contains high concentration of PAM, which should be removed before discharge (Yan et al. 2016).There have been a lot of studies focusing on the biodegradability ...

    Get Price
  • Biodegradation of low molecular weight

    2019-12-12 · We investigated the properties of polymeric systems formed by cross-linking chitosan with modified starch (oxidized maltodextrin). Such a macromolecular cross-linker proved to be efficient to react with chitosan with potentially minimal toxicity. The structural characterization of modified starch alone and of the two-polysaccharide reactive systems was performed using 1H NMR and FTIR. The ...

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  • Polysaccharide Gels Based on Chitosan and Modified

    2020-11-7 · (500 g) were ground to a coarse flour in a hand mill, then extracted by stirring for 1 hour at room temperature with 1,500 ml of sodium chloride solution (1%). After centrifugation 3 J. J. Marshall, and C. M. Lauda (19751, Die Staerke, in press. (27,000 x g…

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  • Purification and properties of phaseolamin, an inhibitor ...

    2016-12-28 · Over the last decades, alginates, natural multifunctional polymers, have increasingly drawn attention as attractive compounds in the biomedical and pharmaceutical fields due to their unique physicochemical properties and versatile biological activities. The focus of the paper is to describe biological and pharmacological activity of alginates and to discuss the present use and future ...

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  • o l o g y : OpenAc h e ces R Rheology: Open Access

    2017-12-29 · Starch-polyacrylamide graft copolymer and polyacrylamide production Starch was gelatinized in respective solvents by heating a starch suspension (6.5% solids) to 90°C and maintaining for 30 min. After gelatinization, the gelatinized starch solution was purged overnight with argon. Acrylamide (1.3 g) was added to the gelatinized starch solution,

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  • Preparation of starch-graft-polyacrylamide

    2003-10-1 · Starch-g-polyacrylamide materials with higher levels of acrylamide were also extruded. The starch feed rate was fixed at 67.5 g/min (dry basis) with a screw speed of 150 rpm. Although the data in Table 2 indicate that higher feed rates could be used, these conditions were selected to reduce the amount of acrylamide handled.

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  • CONFIGURATIONS MAIN CHARACTERISTICS

    2018-11-28 · renewable resources, such as activated starch, to replace traditional polyacrylamide flocculants, as a response to increasing demand from local authorities and industry in this area. This range of biosourced products is perfectly suited for optimal Actiflo operation and …

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  • Relation of Starch Phosphorylases to Starch

    2004-10-15 · Abstract. Tissues of wheat (Triticum aestivum L., var. Star) exhibit three starch phosphorylase activity forms resolved by non-denaturing polyacrylamide gel affinity electrophoresis (P1, P2 and P3).Compartmentation analysis of young leaf tissues showed that P3 is plastidic, whereas P1 and P2 are cytosolic. P1 exhibits a strong binding affinity to immobilized glycogen upon electrophoresis ...

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  • Acrylamide: a Common Food Toxin Related to

    2019-12-6 · Acrylamide and polyacrylamide production operators had hemoglobin AA adducts in the concentration up to 1884 pmol/g, whereas hemoglobin AA adducts level in administrative workers was 97.9 pmol/g (Moorman et al. 2012). It is still unclear whether toxic effects of chronic exposure to acrylamide may accumulate in the organism in the long term.

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  • Concerted Up-regulation of Aldehyde/Alcohol

    2014-7-21 · 15 Aberle T,Burchard W,Radosta S.Starch/Staerke,1994,46:329~335 16 Bello-Perez L A,Paredes-Lopez O,Roger P,Colona P.Cereal Chemistry,1998,75:395~402 17 Hanselmann R,Ehart M,Widmer H M.Starch/Staerke,1995,46:345~349 18 You S G

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  • Frontiers | Soluble Starch Synthase III-1 in

    Indeed, starch is the source of glucose, directly fueling glycolysis to produce ATP under anoxia . We therefore examined the starch content in the cells analyzed above. As shown in Fig. 9A, the amounts of starch in strain CC-124 increased 3–4-fold when zinc was removed from medium, reaching up to 5–6 μg starch/10 6 cells.

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  • Engineering 6-phosphogluconate dehydrogenase

    2020-12-29 · Abstract. Endosperm starch synthesis is a primary determinant of grain yield and is sensitive to high-temperature stress. The maize chloroplast-localized 6-phosphogluconate dehydrogenase (6PGDH), PGD3, is critical for endosperm starch accumulation. Maize also has two cytosolic isozymes, PGD1 and PGD2, that are not required for kernel development.

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  • Invertases involved in the development of the

    2011-2-17 · Frozen tissue (1 g fresh weight) was homogenized at 4 °C (Ultra-Turrax T25, IKA®-Labortechnik, Staufen, Germany) in 6 mL of buffer A and 0.5 g polyvinylpolypyrrolidone (PVPP). Following centrifugation (10 000 g, 15 min, 4 °C), the supernatant underwent a soluble invertase assay. The residue was rinsed with 50 mL of buffer A and the ...

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  • prof amphoteric polyacrylamide-grafted starc

    Preparation of Amphoteric Polyacrylamide-Grafted Starch. 2013-10-01· A polyacrylamide-grafted starch (St-g-AM-DMC-AMPS) flocculant for the coal slurry wastewater was prepared by using corn starch (St), acrylamide (AM), methyl acryloyloxyethy trimethyl ammonium chloride (DMC) as cationic monomer, 2-acrylamido-2-methyl acrylate sulfonic acid (AMPS) as anionic monomer through solution ...

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  • STARCH POLYACRYLAMIDE, POLYACRYLIC ACID

    2021-5-16 · Compound STARCH POLYACRYLAMIDE, POLYACRYLIC ACID SODIUM SALT, GRAFTED 60%with free spectra: 1 FTIR, and 1 Raman.

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  • Variability in waxy (Wx) allele, in-vitro starch ...

    2021-6-8 · Non-denaturing polyacrylamide gel (12%) was prepared by dissolving 29 g of acrylamide and 1 g of N,N′-methylene-bis-acrylamide in 0.5× TBE buffer. Afterwards 20% ammonium persulfate (APS ...

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  • Amphoteric starch derivatives as reusable flocculant for ...

    Amphoteric starch derivatives as reusable flocculant for heavy-metal removal† Liang Wu,a Xingrong Zhang,b Long Chen,a Huan Zhang,a Chengbi Li,b Yin Lv,a Yisheng Xu,ac Xin Jia, a Yulin Shi *a and Xuhong Guo *ac A pH-responsive amphoteric starch derivative (PRAS) bearing dual functional groups (amino and carboxyl

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  • polyacrylamide | eBay

    Polyacrylamide Gel Electrophoresis Lab Techniques - Pharmacia (1986, Paperback) Pre-Owned. C 16.31. Top Rated Seller. Top Rated Seller. or Best Offer. From United States. +C 24.91 shipping estimate. S p o n s o r e d.

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  • PERSONAL CARE - SNF

    2018-7-30 · SNF, headquartered in Andrézieux, France, is a leading global producer of water-soluble polymers with manufacturing sites in Andrézieux, France; Savannah, USA; and Taixing, China. For 2017, SNF had annual revenues of approximately 2.5G€ (2.71B). Over the last 40 years, SNF has developed expertise in polymer chemistries

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  • Accumulation of multiple-repeat starch-binding domains ...

    2015-9-22 · starch-binding domains (SBDs) can be accumulated in starch granules during the starch biosynthesis process, without aVecting the amount of amylose (Ji et al. 2003; Kok-Jacon et al. 2003). It was also shown that more SBD could be incorporated in the granules of the amy-lose-free (amf) potato mutant. One explanation for

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  • Water-Soluble and Biodegradable Pectin-Grafted ...

    2014-4-7 · Pec-g-PAAm.The broad peaks in the range of 3196 to 3322 cm −1 are attributed to the overlapped –OH groups of pectin and symmetric and asymmetric –NH stretching vibrations of polyacrylamide. The peak at 1655 cm −1 accompanied by a shoulder peak at 1610 cm −1 is assigned to amide I and amide II bands, respectively, which confirms the formation of polyacrylamide.

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  • Novel mutant alleles of the starch synthesis gene

    2017-5-8 · Transient starch provides carbon and energy for plant growth, and its synthesis is regulated by the joint action of a series of enzymes. Starch synthesis IV (SSIV) is one of the important starch synthase isoforms, but its impact on wheat starch synthesis has not yet been reported due to the lack of mutant lines. Using the TILLING approach, we identified 54 mutations in the wheat gene TaSSIVb-D ...

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  • Fabrication and photocatalytic performance of

    2020-7-22 · The authors have fabricated novel flower-like Bi 24 O 31 Br 10 micro/nanospheres by a polyacrylamide gel method. The crystal structure, microstructure, bandgap, morphology, and optical property of the as-prepared Bi 24 O 31 Br 10 micro/nanospheres were investigated by multiple techniques. The Bi 24 O 31 Br 10 photocatalyst exhibits excellent photocatalytic performance for the …

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